3-D reconstruction in optical microscopy by a frequency-domain approach
Identifieur interne : 002F50 ( Main/Exploration ); précédent : 002F49; suivant : 002F513-D reconstruction in optical microscopy by a frequency-domain approach
Auteurs : Tullio Tommasi [Italie] ; Alberto Diaspro [Italie] ; Bruno Bianco [Italie]Source :
- Signal Processing [ 0165-1684 ] ; 1991.
Abstract
A general and simple method for 3-D image restoration in optical sectioning microscopy is presented. A set of images taken with an optical system with a known 3-D point spread function (psf) are considered. The restoration task is described, pointing out theoretical constraints on recovering the actual spatial distribution of the original object. A spatial-frequency analysis is performed, using no a priori information about the specimens udner investigation. The feasibility and limitations of 3-D reconstructions are analyzed. Moreover, a procedure is proposed that allows one to recover object projections within an angle range strictly dependent on the psf of the system used. 3-D objects have been simulated in order to produce a set of images similar to those provided by an optical system. Restoration has then been accomplished, and results demonstrate that side-views of an object can be obtained to a satisfactory degree of accuracy. Finally, a discussion concerning the presence of transparent and opaque objects in the field of view is also presented.
Url:
DOI: 10.1016/0165-1684(93)90007-W
Affiliations:
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Bianco, Dipartimento di Ingegneria Biofisica ed Elettronica, Università degli studi di Genova, Via Opera Pia 11a, Genova 16145</wicri:regionArea><wicri:noRegion>Genova 16145</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biophysical and Electronic Engineering, University of Genova, Via Opera Pia 11a, 16145 Geneva</wicri:regionArea><wicri:noRegion>16145 Geneva</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Signal Processing</title><title level="j" type="abbrev">SIGPRO</title><idno type="ISSN">0165-1684</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991">1991</date><biblScope unit="volume">32</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="357">357</biblScope><biblScope unit="page" to="366">366</biblScope></imprint><idno type="ISSN">0165-1684</idno></series><idno type="istex">92FF40958384A237E8E8C69B2F585156373EDB5A</idno><idno type="DOI">10.1016/0165-1684(93)90007-W</idno><idno type="PII">0165-1684(93)90007-W</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-1684</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A general and simple method for 3-D image restoration in optical sectioning microscopy is presented. A set of images taken with an optical system with a known 3-D point spread function (psf) are considered. The restoration task is described, pointing out theoretical constraints on recovering the actual spatial distribution of the original object. A spatial-frequency analysis is performed, using no a priori information about the specimens udner investigation. The feasibility and limitations of 3-D reconstructions are analyzed. Moreover, a procedure is proposed that allows one to recover object projections within an angle range strictly dependent on the psf of the system used. 3-D objects have been simulated in order to produce a set of images similar to those provided by an optical system. Restoration has then been accomplished, and results demonstrate that side-views of an object can be obtained to a satisfactory degree of accuracy. Finally, a discussion concerning the presence of transparent and opaque objects in the field of view is also presented.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Tommasi, Tullio" sort="Tommasi, Tullio" uniqKey="Tommasi T" first="Tullio" last="Tommasi">Tullio Tommasi</name></noRegion><name sortKey="Bianco, Bruno" sort="Bianco, Bruno" uniqKey="Bianco B" first="Bruno" last="Bianco">Bruno Bianco</name><name sortKey="Bianco, Bruno" sort="Bianco, Bruno" uniqKey="Bianco B" first="Bruno" last="Bianco">Bruno Bianco</name><name sortKey="Diaspro, Alberto" sort="Diaspro, Alberto" uniqKey="Diaspro A" first="Alberto" last="Diaspro">Alberto Diaspro</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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